Note that traditional desktop applications don’t appear on this list. Under “Choose which apps can access your camera,” ensure the app that wants to access your camera isn’t listed and set to “Off.” If it appears in this list, set it to “On.” Previously, it only affected UWP apps and didn’t affect traditional desktop applications.
This option was changed with the release of Windows 10’s April 2018 Update. However, the Windows operating system can still use your camera for features like Windows Hello. Just under that, ensure “Allow apps to access your camera” is also set to “On.” If it’s set to Off, no applications on your system-including desktop applications-can see or use your camera.